基于EST-SSR標(biāo)記的沙棘品種鑒定及指紋圖譜構(gòu)建
生態(tài)與農(nóng)村環(huán)境學(xué)報(bào)
頁(yè)數(shù): 12 2023-04-13
摘要: 以沙棘(Hippophae rhamnoides)優(yōu)良品種“實(shí)優(yōu)1號(hào)”為材料,對(duì)其葉片進(jìn)行轉(zhuǎn)錄組測(cè)序,利用微衛(wèi)星識(shí)別軟件(microsatellite identification tool, MISA)和Primer 3(version 2.3.4)對(duì)獲得的序列進(jìn)行簡(jiǎn)單重復(fù)序列(simple sequence repeat, SSR)位點(diǎn)挖掘和引物設(shè)計(jì),以收集的42份沙棘品種... (共12頁(yè))